1| Incubate the cell lysate with 50 μl of a 50% slurry of glutathione-agarose beads and 25 μg of GST for 2 h at 4 °C with end-over-end mixing. The amount of lysate needed to detect an interaction is ...

You have full access to this article via your institution. Serial dilutions were performed using DNA extracted from Nras mutation-bearing cell lines into placental DNA (wild-type). The assay ...